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Gene profile in the spleen under massive partial hepatectomy using complementary DNA microarray and pathway analysis.

Yusuke Arakawa1, Mitsuo Shimada, Tohru Utsunomiya

  • 1Department of Surgery, Institute of Health Biosciences, The University of Tokushima, Tokushima, Japan.

Journal of Gastroenterology and Hepatology
|March 18, 2014
PubMed
Summary

Partial hepatectomy (Hx) in rats upregulates inflammatory genes in the spleen, including chemokine and early growth response genes. This suggests the spleen may negatively impact recovery after Hx.

Keywords:
EGR1GRO1massive hepatectomymicroarraysplenectomy

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Area of Science:

  • Gastroenterology
  • Immunology
  • Molecular Biology

Background:

  • The spleen's role in hepatic function after partial hepatectomy (Hx) or liver transplantation is not fully understood.
  • Splenectomy can improve liver function in specific clinical scenarios, suggesting a complex interaction.

Purpose of the Study:

  • To investigate the spleen's gene expression profile following 90% Hx in rats.
  • To elucidate the molecular mechanisms underlying spleen changes post-Hx.

Main Methods:

  • Complementary DNA microarray analysis of rat spleen mRNA at 0, 3, and 6 hours post-90% Hx.
  • Pathway analysis using Ingenuity Pathway Analysis (IPA) to interpret gene expression data.

Main Results:

  • Upregulation of chemokine activity-related genes (e.g., Cxcl1/GRO1, Cxcl2/MIP-2) in the spleen post-Hx.
  • Upregulation of immediate early response genes (e.g., EGR1, FOS, ATF3) in the spleen post-Hx.
  • Defined cut-off values for gene expression changes ( >2.0-fold or <0.5-fold).

Conclusions:

  • Significant expression of inflammatory-related genes occurs in the spleen after 90% Hx.
  • The spleen may exert a detrimental effect during the post-Hx phase due to induced chemokine and transcription factors like GRO1 and EGR1.