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Related Concept Videos

Types Of Column Chromatography01:29

Types Of Column Chromatography

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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Upstream Processing01:27

Upstream Processing

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Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
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Affinity Chromatography01:03

Affinity Chromatography

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

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Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
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Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

3.0K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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Laboratory Scale Production and Purification of a Therapeutic Antibody
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Purifying biopharmaceuticals: knowledge-based chromatographic process development.

Alexander T Hanke1, Marcel Ottens1

  • 1Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.

Trends in Biotechnology
|March 18, 2014
PubMed
Summary
This summary is machine-generated.

Biopharmaceutical purification, often a manufacturing bottleneck, requires systematic strategies. Advances in understanding solute properties and resin interactions optimize liquid chromatography for efficient downstream process development.

Keywords:
chromatographyhigh-throughputhost cell proteinsmathematical modellingprocess development

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Area of Science:

  • Biopharmaceutical Manufacturing
  • Process Development
  • Chromatography

Background:

  • Biopharmaceutical purification is a critical bottleneck in manufacturing.
  • Increasing product diversity and regulatory pressures necessitate improved process development strategies.
  • Liquid chromatography is central to purification but its understanding is underutilized.

Purpose of the Study:

  • To review advances in determining solute properties and resin interactions.
  • To outline the application of this knowledge in downstream process development.
  • To promote rational and systematic strategies for bioprocess optimization.

Main Methods:

  • Overview of advancements in property and interaction measurement techniques.
  • Analysis of how these measurements inform process design.
  • Integration of knowledge across downstream process development stages.

Main Results:

  • Improved methods for characterizing mixtures, solutes, and resin interactions.
  • Demonstration of how this knowledge rationalizes process development choices.
  • A framework for systematic and generally applicable downstream process development.

Conclusions:

  • Understanding molecular properties and interactions is key to overcoming purification bottlenecks.
  • Rational application of this knowledge enhances efficiency and predictability in biopharmaceutical manufacturing.
  • Systematic process development strategies are essential for meeting modern biopharmaceutical demands.