Related Experiment Video
Updated: May 2, 2026
Isolation of Pulmonary Artery Smooth Muscle Cells from Neonatal Mice
Published on: October 19, 2013
The effects of resveratrol on hyperoxia-induced lung injury in neonatal rats
Özmert M A Özdemir1, Ersin Gözkeser1, Ferda Bir2
1Department of Pediatrics, Faculty of Medicine, Pamukkale University, Denizli, Turkey.
Insights
Resveratrol, an antioxidant, shows promise in preventing lung injury in newborn rats exposed to high oxygen levels. This study demonstrates its potential to reduce inflammation and oxidative stress, key factors in bronchopulmonary dysplasia.
Area of Science:
- Neonatal physiology
- Pulmonary medicine
- Pharmacology
Background:
- Bronchopulmonary dysplasia (BPD) is a significant cause of illness and death in premature infants.
- Inflammation and oxidative injury are critical in BPD development.
- Resveratrol possesses antioxidant and anti-inflammatory properties.
Purpose of the Study:
- To investigate the effects of resveratrol on a hyperoxia-induced lung injury model in newborn rats.
- To assess the histopathological and biochemical impacts of resveratrol treatment.
Main Methods:
- Newborn rats were exposed to hyperoxia from postnatal day 3 to 13.
- Rats were divided into four groups: air-exposed (control) and hyperoxia-exposed, with or without resveratrol treatment (30 mg/kg/day).
- Lung tissue was analyzed for alveolar surface area, fibrosis, smooth muscle actin (SMA), glutathione (GSH), superoxide dismutase (SOD), nitric oxide (NO), tumor necrosis factor-α (TNF-α), and nuclear factor kappa B (NF-κB).
Main Results:
- Hyperoxia significantly increased alveolar surface area, fibrosis, SMA score, and NO levels.
- Resveratrol treatment in hyperoxia-exposed rats reduced SMA score, NO, and TNF-α levels.
- Resveratrol administration increased GSH and SOD levels in hyperoxia-exposed rats.
Conclusions:
- Oxidative stress and nitric oxide play a role in hyperoxia-induced lung injury.
- Resveratrol demonstrates a protective effect against hyperoxic lung injury.
- Resveratrol's benefits are attributed to its anti-inflammatory and antioxidant actions.
Background:
Bronchopulmonary dysplasia (BPD) is a chronic lung disease that causes significant morbidity and mortality in premature infants. Inflammation and oxidative injury play an important role in the pathogenesis of BPD. Resveratrol is an antioxidant and anti-inflammatory agent. In this study, the histopathological and biochemical effects of resveratrol on a hyperoxia-induced lung injury model in newborn rats were investigated.
Methods:
The experiment was performed on newborn rat pups from the 3(rd) to 13(th) postnatal day and they were randomly divided into four groups: Group 1 (air-exposed + saline, n = 10), Group 2 (air-exposed + resveratrol, n = 11), Group 3 (hyperoxia-exposed + saline, n = 6) and Group 4 (hyperoxia-exposed + resveratrol, n = 7). Resveratrol was administered (30 mg/kg/day) intraperitoneally. The histopathological effects of resveratrol on lung tissue were assessed by alveolar surface area, fibrosis, and smooth muscle actin (SMA) score, and the biochemical effects on lung tissue were assessed by glutathione (GSH), superoxide dismutase (SOD), nitric oxide (NO), tumor necrosis factor-α (TNF-α), and nuclear factor kappa B (NF-κB) levels.
Results:
The alveolar surface area, fibrosis, SMA score, and NO levels were found to be significantly higher in Group 3 compared with Group 1 (p < 0.05). In addition, it was found that resveratrol treatment significantly reduced the SMA score and the NO and TNF-α levels, and increased the GSH and SOD levels in the hyperoxia group (p < 0.05).
Conclusion:
This experimental study showed that oxidative stress and NO contributed to the pathogenesis of hyperoxia-induced lung injury, and that resveratrol had a preventive effect on hyperoxic lung injury through its anti-inflammatory and antioxidant properties.
Related Concept Videos
01:23Physiological Control of Respiration
Breathing, a seemingly passive process, is regulated by the respiratory center in the brainstem. This center coordinates the involuntary control of respirations, which means it occurs without conscious effort, ensuring a smooth and uninterrupted pattern.
Regulation of Ventilation
The body maintains ventilation by monitoring levels of carbon dioxide (CO2), oxygen (O2), and hydrogen ion concentration (pH) in the arterial blood. Among these factors, the level of CO2 plays a crucial...
01:19Respiratory Assessment: Purpose and Indications
Objectives and Importance:
The primary goal of respiratory assessment is to evaluate patients at early risk of clinical deterioration. Since respiratory distress often precedes other signs of declining health, breathing patterns and sounds become a...