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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
Published on: May 4, 2012
[Enzyme linked counter reference electrophoresis assay and reverse passive hemagglutination assay in general survey
Abstract:
The AFP content in sera of 11735 persons was tested by the new method-enzyme linked counter reference electrophoresis assay and the conventional method-reverse passive hemagglutination assay (RPHA). AFP level in 15 out of 11735 persons, determined by the former technique, was over the normal range, quite similar to those by radiorocket electrophoresis. A good correlation was noted between the two techniques. But by RPHA, AFP was positive only in 7/15 and gave a false positive up to 4.53% (532/11735). It is indicated that the new method is more specific and sensitive than RPHA, and more suitable for general survey of liver cancer.
