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Oncogene Expression Analysis with Alterations in pH in a Pancreatic Ductal Cell Line
Published on: April 11, 2025
978
Proliferating cell unclear antigen-associated factor (PAF15): a novel oncogene
Chanlu Xie1, Mu Yao2, Qihan Dong3
1School of Science and Health, The University of Western Sydney, Australia.
The International Journal of Biochemistry & Cell Biology
|March 18, 2014
Summary
Proliferating cell nuclear antigen (PCNA)-Associated Factor (PAF15) is crucial for DNA repair and cell survival. Its overexpression enhances DNA damage repair and tumor growth, suggesting PAF15 as a cancer therapeutic target.
Area of Science:
- Molecular Biology
- Cell Biology
- Cancer Research
Background:
- Proliferating cell nuclear antigen (PCNA)-Associated Factor (PAF15) is a protein involved in DNA replication and repair.
- PAF15 interacts with PCNA and ubiquitin enzymes, playing a role in DNA polymerase recruitment.
Purpose of the Study:
- To investigate the role of PAF15 in DNA damage response and its implications in cancer.
- To explore PAF15 as a potential therapeutic target for cancer treatment.
Main Methods:
- Studied the interaction of PAF15 with PCNA and its role in DNA repair mechanisms.
- Investigated the effects of PAF15 overexpression and attenuation on DNA replication, cell survival, and DNA damage repair.
- Examined the impact of ectopic PAF15 expression on tumor formation in mouse models.
- Analyzed PAF15 expression levels in human malignancies.
Main Results:
- PAF15 facilitates DNA replicative polymerase recruitment via PCNA ubiquitination.
- PAF15 regulates the switch to translesion synthesis polymerase under DNA damage.
- Overexpression of PAF15 promotes UV-induced DNA damage repair, cell survival, and tumorigenicity.
- Reduced PAF15 levels impair DNA replication and cell survival.
- Elevated PAF15 expression correlates with poor prognosis in human cancers.
Conclusions:
- PAF15 is a key regulator of DNA damage response and cell proliferation.
- Aberrant PAF15 expression contributes to carcinogenesis.
- PAF15 represents a potential therapeutic target for cancer treatment.
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