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Study of carD gene sequence in clinical isolates of Mycobacterium tuberculosis

Hossein Sarmadian1, Razieh Nazari2, Mohammad Reza Zolfaghari2

  • 1Arak University of Medical Sciences Tuberculosis and Pediatric Infectious Research Center Arak Iran.

Acta Microbiologica Et Immunologica Hungarica
|March 18, 2014
PubMed

Insights

The carD gene is crucial for Mycobacterium tuberculosis survival and growth. Sequencing revealed its conserved nature in drug-susceptible and resistant strains, making it a target for new TB drugs and rapid diagnostics.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Mycobacterium tuberculosis (MTB) growth is linked to rRNA transcription, regulated by the carD gene.
  • Understanding carD gene variations is essential for developing new anti-tuberculosis strategies.

Purpose of the Study:

  • To sequence the carD gene in drug-susceptible and resistant M. tuberculosis clinical isolates.
  • To design a PCR assay for rapid detection of M. tuberculosis using the carD gene.

Main Methods:

  • DNA extraction from 41 M. tuberculosis clinical isolates with varying drug resistance patterns.
  • PCR amplification of the entire carD gene using specifically designed primers.
  • Sequencing of PCR products using the ABI system and analysis of nucleotide and amino acid sequences.

Main Results:

  • A 524 bp fragment of the carD gene was successfully amplified and sequenced.
  • Sequence analysis indicated that the carD gene is highly conserved across all investigated M. tuberculosis isolates, including drug-resistant strains.
  • The TRCF domain within the N-terminal region of the CarD protein was found to be fully conserved.

Conclusions:

  • The carD gene's high conservation in M. tuberculosis makes it a promising target for novel anti-tuberculosis drug development.
  • A PCR-based detection method targeting the carD gene is recommended for rapid and accurate diagnosis of M. tuberculosis in clinical settings.

Keywords:
Mycobacterium tuberculosiscarD genesequence

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