Elevated triglycerides may affect cystatin C recovery
Samantha H Witzel1, Katherine Butts2, Guido Filler2
1Department of Paediatrics, Children's Hospital, London Health Sciences Centre, University of Western Ontario, London, Ontario N6A 5W9, Canada.
Clinical Biochemistry
|March 18, 2014
Summary
High triglyceride levels significantly lower cystatin C (CysC) measurements. A developed formula can correct CysC values in samples with high lipid content, improving accuracy for kidney function assessment.
Area of Science:
- Clinical Chemistry
- Nephrology
- Biomarker Analysis
Background:
- Cystatin C (CysC) is a biomarker for kidney function.
- High triglyceride concentrations can interfere with laboratory assays.
Purpose of the Study:
- To investigate the impact of triglyceride concentration on CysC measurements.
- To develop a method for correcting CysC values affected by lipids.
Main Methods:
- Serum samples from nephrology patients were processed to create varying triglyceride concentrations.
- Regression analysis was used to derive a correction formula.
- The formula was validated on a separate set of samples.
Main Results:
- A significant inverse correlation was found between triglyceride levels and measured CysC (r=-0.56, p<0.0001).
- A correction formula was developed and validated, showing reduced bias in CysC measurements.
Conclusions:
- Triglyceride concentration significantly affects CysC measurements.
- The developed formula can correct for triglyceride interference in CysC assays, especially when air centrifugation is insufficient.
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