Circular permutation of chicken interleukin-1 beta enhances its thermostability
Wen-Ting Chen1, Ting Chen, Chao-Sheng Cheng
1Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, Taiwan. hstin@mx.nthu.edu.tw.
Chemical Communications (Cambridge, England)
|March 18, 2014
Abstract:
Interleukin-1β is a cytokine critically involved in immune and inflammatory responses. To extend its use as a component of avian vaccines, a circularly permuted chicken interleukin-1β was synthesized that maintains its activity after pre-incubation at high temperatures, unlike wild-type chicken interleukin-1β, which is irreversibly inactivated at high temperatures.
