Related Experiment Video
Updated: May 2, 2026

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
Published on: August 27, 2021
Bacteriophage vehicles for phage display: biology, mechanism, and application
Walead Ebrahimizadeh1, Masoumeh Rajabibazl
1Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran, W_Ebrahimizadeh@hotmail.com.
Abstract:
The phage display technique is a powerful tool for selection of various biological agents. This technique allows construction of large libraries from the antibody repertoire of different hosts and provides a fast and high-throughput selection method. Specific antibodies can be isolated based on distinctive characteristics from a library consisting of millions of members. These features made phage display technology preferred method for antibody selection and engineering. There are several phage display methods available and each has its unique merits and application. Selection of appropriate display technique requires basic knowledge of available methods and their mechanism. In this review, we describe different phage display techniques, available bacteriophage vehicles, and their mechanism.
Related Concept Videos
DNA Bacteriophages
Lytic Cycle of Bacteriophages
Lysogenic Cycle of Bacteriophages
Viral Replication: Lytic Cycle
Viral Replication: Lysogenic Cycle

