Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
MicroRNAs01:22

MicroRNAs

21.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HSV-2 gC2 mRNA immunization in mice protects by producing antibodies that bind immune evasion epitopes.

PLoS pathogens·2026
Same author

HSV-2 gE2/gI2 are immune evasion molecules that bind IgG Fc to inhibit antibody-dependent cellular cytotoxicity.

Frontiers in immunology·2026
Same author

The human cytomegalovirus chemokine binding protein UL22A is necessary for efficient reactivation from latency in CD34 <sup>+</sup> hematopoietic progenitor cells and humanized mice.

bioRxiv : the preprint server for biology·2026
Same author

Unveiling the unique interaction mechanism of herpes simplex virus 2 glycoprotein C with C3b.

bioRxiv : the preprint server for biology·2025
Same author

Human cytomegalovirus UL78 is a nuclear-localized GPCR necessary for efficient reactivation from latent infection in CD34<sup>+</sup> hematopoietic progenitor cells.

Journal of virology·2025
Same author

Cytomegalovirus latency-the sum of subtleties.

Journal of virology·2025

Related Experiment Video

Updated: May 2, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

555

Techniques for characterizing cytomegalovirus-encoded miRNAs.

Lauren M Hook1, Igor Landais, Meaghan H Hancock

  • 1Vaccine & Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 19, 2014
PubMed
Summary

MicroRNAs (miRNAs) regulate gene expression post-transcriptionally. This chapter details methods for expressing, detecting, inhibiting miRNAs, and identifying their mRNA targets.

More Related Videos

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.2K
MicroRNA-based Regulation of Picornavirus Tropism
09:05

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

7.1K

Related Experiment Videos

Last Updated: May 2, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
09:06

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

555
mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

2.2K
MicroRNA-based Regulation of Picornavirus Tropism
09:05

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

7.1K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are small noncoding RNAs regulating gene expression post-transcriptionally.
  • miRNAs function by binding to 3' untranslated regions of messenger RNA (mRNA) transcripts.
  • The novel mechanism of miRNA-mediated gene regulation requires specialized characterization techniques.

Purpose of the Study:

  • To review current techniques for miRNA research.
  • To cover methods for miRNA expression, detection, and inhibition.
  • To discuss strategies for identifying miRNA targets.

Main Methods:

  • Review of established molecular biology techniques.
  • Description of experimental protocols for miRNA manipulation.
  • Explanation of target identification methodologies.

Main Results:

  • Comprehensive overview of available miRNA research tools.
  • Detailed discussion of techniques for analyzing miRNA function.
  • Guidance on selecting appropriate methods for specific research questions.

Conclusions:

  • A wide array of techniques exists to study miRNAs.
  • Understanding these methods is crucial for advancing miRNA research.
  • This chapter serves as a guide to miRNA characterization and target identification.