Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Comparison of Cannulation Strategies for Minimally Invasive Mitral Valve Surgery.

Interdisciplinary cardiovascular and thoracic surgery·2026

Same author

Spatiotemporal trends of ambient radiofrequency electromagnetic fields (RF-EMF) during the 5G rollout in Switzerland.

Journal of exposure science & environmental epidemiology·2026

Same author

Ten-year outcomes of pulmonary endarterectomy in Switzerland: The Zurich experience.

JTCVS open·2026

Same author

Cosmological Magnetic Fields from Ultralight Dark Matter.

Physical review letters·2026

Same author

Right Ventricular Outflow Tract Diameter for Event Prediction in Arrhythmogenic Right Ventricular Cardiomyopathy: Incremental Value Over Echocardiographic Free-Wall Strain.

JACC. Cardiovascular imaging·2025

Same author

Behaviour and reproduction of Drosophila melanogaster exposed to 3.6 GHz radio-frequency electromagnetic fields.

PloS one·2025

Search research articles

Related Experiment Video

Updated: May 2, 2026

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

Published on: December 13, 2012

Impedance flow cytometry gauges proliferative capacity by detecting TRPC1 expression.

Sara Crocetti¹, Christian Beyer, Silvio Unternährer

¹Institute for Biomechanics, ETH Zürich, Switzerland.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|March 19, 2014

Summary

Impedance-based flow cytometry (IFC) detects the functional expression of TRPC1 channels in stem cells. This non-invasive method identifies early-stage stem cell expansion by sensing membrane changes, not calcium flow.

Keywords:

calcium flux cancer cells cell cycle flow cytometry muscle myoblasts progenitor cells stem cells tissue regeneration transient receptor potential channels (TRP)

More Related Videos

Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry

Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry

Published on: December 16, 2022

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

Published on: April 19, 2017

Related Experiment Videos

Last Updated: May 2, 2026

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

Published on: December 13, 2012

Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry

Analysis of T-cell Receptor-Induced Calcium Influx in Primary Murine T-cells by Full Spectrum Flow Cytometry

Published on: December 16, 2022

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry

Published on: April 19, 2017

Area of Science:

Biophysics
Cell Biology
Biotechnology

Background:

Mechanically-regulated calcium entry facilitates stem cell expansion and directs development toward mechanosensitive tissues.
Transient receptor potential C (TRPC) channels are key conduits for calcium entry into proliferating myoblasts.
Non-invasive methods to study this calcium pathway are limited.

Purpose of the Study:

To develop and validate a high-throughput method for detecting TRP channel expression in cells.
To investigate the correlation between impedance-based flow cytometry (IFC) signals and TRPC1 channel activity.
To assess IFC's potential for non-invasively identifying early-stage stem cell expansion.

Main Methods:

Utilized a microfluidic configuration of impedance-based flow cytometry (IFC).
Detected TRP channel expression and correlated IFC signal changes with functional TRPC1 channel expression and cell proliferation.
Examined the effects of pharmacological agents, mechanical conditions, and malignant states on TRPC1 expression and IFC signals.

Main Results:

IFC successfully detected TRP channel expression at high throughput.
Changes in the IFC signal correlated with functional TRPC1 channel expression and cell proliferation.
IFC signals reflected alterations in TRPC1 channel expression due to various stimuli but not cation permeation changes.
Results suggest IFC detects membrane organization changes linked to TRPC1 activation and surface expression.

Conclusions:

IFC provides a novel, non-invasive method to detect functional TRPC1 channel expression in proliferating cells.
IFC technology can identify changes in cell membrane organization associated with TRPC1 channel activity.
IFC holds potential for identifying living stem cells during early expansion stages without staining or fixation.