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Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
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[Bactericidal effect of lysozyme].

V M Podborodov, M Iu Shchelkanov, I P Smirnova

    Antibiotiki I Khimioterapiia = Antibiotics and Chemoterapy [Sic]
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    Summary

    Ultrasound effectively isolates potent lysozyme from tick hemolymph, showing superior bactericidal activity against Staphylococcus aureus and Micrococcus luteus compared to conventional methods.

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    Area of Science:

    • Zoology
    • Biochemistry
    • Microbiology

    Context:

    • Lysozymes are crucial antimicrobial enzymes found in various organisms.
    • Tick hemolymph is a potential source for novel antimicrobial agents.
    • Ultrasound-assisted extraction offers a novel method for enzyme isolation.

    Purpose:

    • To isolate and characterize lysozyme from the hemolymph of Alveonasus lahorensis and Hyalomma marginatum ticks.
    • To evaluate the bactericidal efficacy of ultrasound-extracted tick lysozyme against pathogenic bacteria.
    • To compare the activity of ultrasound-extracted lysozyme with conventional sources.

    Summary:

    • Lysozyme was successfully isolated from Alveonasus lahorensis and Hyalomma marginatum hemolymph using ultrasound.
    • Ultrasound-extracted tick lysozyme demonstrated significantly enhanced bactericidal effects against Staphylococcus aureus and Micrococcus luteus.
    • Disintegration of hemolymph cells via ultrasound treatment boosted lysozyme production.

    Impact:

    • This study highlights ultrasound-assisted extraction as an efficient method for obtaining highly active lysozyme from ticks.
    • The findings suggest tick lysozyme as a promising alternative antimicrobial agent for combating bacterial infections.
    • Enhanced lysozyme production through cell disintegration offers potential for scalable antimicrobial solutions.