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Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
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Relaxin and Sertoli cell proliferation.

Aline R Nascimento, Maristela T Pimenta, Thais F G Lucas

    Italian Journal of Anatomy and Embryology = Archivio Italiano Di Anatomia Ed Embriologia
    |March 20, 2014
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    Relaxin and follicle-stimulating hormone (FSH) impact immature rat Sertoli cell proliferation via opposing signaling pathways. Relaxin activates proliferation, while FSH inhibits it, suggesting a complex interplay for cell development.

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    Area of Science:

    • Reproductive biology
    • Cell signaling
    • Endocrinology

    Background:

    • Immature Sertoli cells are crucial for spermatogenesis and their proliferation is influenced by hormones like FSH, testosterone, and estradiol.
    • Understanding the molecular mechanisms regulating Sertoli cell proliferation is vital for reproductive health research.

    Purpose of the Study:

    • To investigate the distinct and potentially opposing effects of relaxin and FSH on Sertoli cell proliferation and signaling pathways.
    • To elucidate the roles of MEK/ERK1/2, PI3K/AKT, and cAMP pathways in mediating these hormonal effects.

    Main Methods:

    • Primary culture of Sertoli cells isolated from 15-day old Wistar rats.
    • Treatment with relaxin and follicle-stimulating hormone (FSH).
    • Analysis of MEK/ERK1/2, PI3K/AKT phosphorylation, and cAMP production.

    Main Results:

    • Relaxin significantly stimulated Sertoli cell proliferation by activating MEK/ERK1/2 and PI3K/AKT pathways.
    • FSH inhibited both ERK1/2 and AKT phosphorylation, while strongly increasing cAMP production.
    • Relaxin inhibited basal cAMP production, contrasting with FSH's effect.

    Conclusions:

    • Relaxin and FSH exert opposing effects on key signaling pathways (MEK/ERK1/2, PI3K/AKT, cAMP) in immature rat Sertoli cells.
    • The interplay between relaxin and FSH is critical for regulating Sertoli cell proliferation and differentiation.
    • These findings offer insights into hormonal control of testicular development.