Related Experiment Video
Updated: Feb 7, 2026
08:55
Author Spotlight: Harnessing DNA Barcode Technology to Enhance the Efficiency of Medicinal Plant Identification
Published on: November 1, 2024
2.5K
Next-generation DNA barcoding: using next-generation sequencing to enhance and accelerate DNA barcode capture from
Shadi Shokralla1, Joel F Gibson, Hamid Nikbakht
1Department of Integrative Biology, Biodiversity Institute of Ontario, University of Guelph, 50 Stone Road East, Guelph, ON, Canada, N1G 2W1; Department of Microbiology, Mansoura University, Egypt, 35516.
Molecular Ecology Resources
|March 20, 2014
Summary
Next-generation sequencing offers a powerful, cost-effective method for DNA barcoding, enabling simultaneous analysis of hundreds of specimens and overcoming limitations of traditional Sanger sequencing for species identification.
Area of Science:
- Genomics and Molecular Biology
- Bioinformatics and Computational Biology
Background:
- DNA barcoding is crucial for specimen identification and discovering new species.
- Traditional Sanger sequencing faces challenges like high costs, low throughput, and difficulties with complex samples (pseudogenes, symbionts, heteroplasmy).
Purpose of the Study:
- To evaluate the application of next-generation sequencing (NGS) for high-throughput DNA barcoding.
- To demonstrate parallel acquisition of DNA barcode sequences from numerous specimens simultaneously.
Main Methods:
- Specimens were tagged with unique 10-mer oligonucleotides during PCR amplification.
- 454 pyrosequencing was used to generate DNA barcodes from 190 specimens.
- Analysis focused on recovering full-length barcodes and identifying potential sequencing issues.
Main Results:
- NGS enabled simultaneous DNA barcoding of hundreds of specimens with high efficiency.
- An average of 143 sequence reads were obtained per specimen, yielding full-length barcodes for most individuals.
- NGS successfully detected Wolbachia, nontarget species, and heteroplasmic sequences, providing richer data.
Conclusions:
- Next-generation sequencing platforms are highly valuable for large-scale DNA barcoding projects.
- NGS offers protocol simplicity, reduced cost per read, faster throughput, and enhanced information content compared to Sanger sequencing.
Related Concept Videos
03:00Next-generation Sequencing
98.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
98.6K
02:57DNA-only Transposons
17.5K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
17.5K
02:40DNA Replication
59.6K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied. After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
Replication in Prokaryotes
DNA replication...
59.6K
01:09Recombinant DNA
103.3K
Overview
103.3K
03:06From DNA to Protein
22.4K
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
22.4K
00:55DNA Helicases
24.2K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
24.2K