Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Cardiac and Thrombotic Effects of Severe Carbon Monoxide Poisoning: A Case Series.

Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc·2026

Same author

Side Effects of Hyperbaric Oxygen Therapy.

Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc·2026

Same author

Myelin Basic Protein Expressing Microparticles Predict Neurologic Morbidity Risk From Acute Carbon Monoxide Poisoning.

Critical care medicine·2026

Same author

Laptop-style point-of-care ultrasound machines in the multiplace hyperbaric oxygen treatment chamber.

Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc·2026

Same author

Hyperbaric oxygen therapy for selected problem wounds.

Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc·2026

Same author

Intravenous Thrombolysis Is Associated With Better Outcomes in Large-Vessel Occlusion Requiring Endovascular Therapy.

Stroke (Hoboken, N.J.)·2026

Search research articles

Related Experiment Video

Updated: May 2, 2026

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Published on: April 6, 2022

CD34+/CD45-dim stem cell mobilization by hyperbaric oxygen - changes with oxygen dosage.

Marvin Heyboer¹, Tatyana N Milovanova², Susan Wojcik¹

¹Department of Emergency Medicine, State University of New York Upstate Medical University, Syracuse, NY, USA.

Stem Cell Research

|March 20, 2014

Summary

Hyperbaric oxygen therapy at 2.5 atmospheres absolute (ATA) mobilizes significantly more stem cells than 2.0 ATA. This higher mobilization efficiency with increased oxygen partial pressure is crucial for regenerative medicine applications.

More Related Videos

Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles

Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles

Published on: December 23, 2022

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

Published on: November 5, 2019

Related Experiment Videos

Last Updated: May 2, 2026

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Published on: April 6, 2022

Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles

Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles

Published on: December 23, 2022

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

Published on: November 5, 2019

Area of Science:

Regenerative Medicine
Hyperbaric Medicine
Stem Cell Biology

Background:

Hyperbaric oxygen treatment (HBOT) mobilizes stem/progenitor cells via free radical mechanisms.
Understanding optimal oxygen partial pressures for HBOT-induced stem cell mobilization is critical.

Purpose of the Study:

To investigate the hypothesis that different oxygen partial pressures influence stem/progenitor cell mobilization efficiency.
To compare the efficacy of 2.0 ATA versus 2.5 ATA HBOT protocols on stem cell mobilization.

Main Methods:

Blood samples collected from 20 patients before and after HBOT (1st, 10th, 20th treatments).
Two HBOT protocols used: 2.0 ATA and 2.5 ATA oxygen exposure.
Quantification of CD34+, CD45-dim leukocytes and intracellular regulatory proteins (HIFs, thioredoxin-1, PARP) in mobilized cells.

Main Results:

Post-treatment CD34+, CD45-dim leukocyte counts were consistently 2-fold higher than pre-treatment values for both protocols.
The 2.5 ATA protocol resulted in significantly greater cell mobilization (1.9 to 3-fold) compared to 2.0 ATA after the 10th and 20th treatments.
Mobilized cells from both protocols showed doubled intracellular concentrations of HIFs, thioredoxin-1, and PARP, with no significant difference between pressure groups.

Conclusions:

Stem/progenitor cell mobilization is more efficient with 2.5 ATA compared to 2.0 ATA HBOT.
All newly mobilized cells exhibit elevated levels of key regulatory proteins, irrespective of the oxygen pressure used.