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Updated: Sep 14, 2026
Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
Published on: September 20, 2016
Mutation analysis of components of the Mediator kinase module in MED12 mutation-negative uterine leiomyomas
N Mäkinen1, H-R Heinonen1, J Sjöberg2
1Department of Medical Genetics, Genome-Scale Biology Research Program, University of Helsinki, PO Box 63, 00014 Helsinki, Finland.
Background:
Kinase module of Mediator complex ('CDK8 submodule') consists of four subunits: CDK8, Cyclin C, MED12, and MED13. Recently, we reported recurrent MED12 mutations in 70% of uterine leiomyomas. The aim of this study was to analyse whether mutations in other components of the module contribute to the development of these lesions.
Methods:
Mutation screening of altogether 70 MED12 mutation-negative uterine leiomyomas was carried out by direct sequencing.
Results:
None of the tumours displayed somatic mutations in the coding regions of CDK8/CDK19, CCNC, or MED13.
Conclusions:
Mutations in CDK8/CDK19, CCNC, and MED13 do not frequently contribute to genesis of uterine leiomyomas.
