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Sudan black B positivity in acute lymphoblastic leukemia
B S Charak1, S H Advani, S M Karandikar
1Department of Medical Oncology, Tata Memorial Hospital, Bombay, India.
Acta Haematologica
|January 1, 1988
Summary
Sudan black B (SBB) positivity in acute lymphoblastic leukemia (ALL) blast cells is rare and not specific for myeloid lineage. This finding suggests SBB staining alone is insufficient for diagnosing myeloid leukemia in ALL cases.
Area of Science:
- Hematology
- Oncology
- Clinical Pathology
Background:
- Acute lymphoblastic leukemia (ALL) is a heterogeneous lymphoid malignancy.
- Accurate immunophenotypic and cytochemical classification is crucial for ALL diagnosis and treatment.
- Sudan black B (SBB) is a stain traditionally associated with myeloid differentiation.
Purpose of the Study:
- To investigate the occurrence and significance of Sudan black B (SBB) positivity in acute lymphoblastic leukemia (ALL).
- To determine if SBB reactivity in ALL blast cells indicates myeloid lineage or is a non-specific finding.
Main Methods:
- Retrospective review of 458 patient records diagnosed with ALL.
- Diagnosis confirmed by clinical, morphological, cytochemical, and immunophenotypic analyses.
- Evaluation of blast cell reactivity with Sudan black B (SBB) and other myeloid markers.
Main Results:
- Six out of 458 (1.3%) ALL patients exhibited SBB-positive blast cells.
- These SBB-positive ALL cases lacked reactivity with other myeloid markers.
- The presence of SBB positivity did not correlate with other myeloid-specific markers.
Conclusions:
- Sudan black B (SBB) positivity can occur in a small subset of acute lymphoblastic leukemia (ALL) cases.
- SBB reactivity in ALL is not indicative of myeloid lineage.
- SBB staining should not be considered a specific marker for myeloid differentiation in the context of ALL diagnosis.