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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
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Complete genome sequence of the Pectobacterium carotovorum subsp. carotovorum virulent bacteriophage PM1

Jeong-A Lim1, Hakdong Shin, Dong Hwan Lee

  • 1Microbial Safety Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Korea.

Archives of Virology
|March 20, 2014
PubMed

Insights

A novel virulent bacteriophage, PM1, was isolated and characterized. This Myoviridae family phage infects Pectobacterium carotovorum subsp. carotovorum and exhibits T7-like characteristics, confirming its virulent nature.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Pectobacterium carotovorum subsp. carotovorum causes significant plant diseases.
  • Bacteriophages are viruses that infect bacteria and hold potential for biocontrol.
  • Understanding bacteriophage genomes is crucial for their application.

Purpose of the Study:

  • To isolate and characterize a novel virulent bacteriophage infecting Pectobacterium carotovorum subsp. carotovorum.
  • To determine the genomic and morphological features of the isolated bacteriophage.
  • To assess the potential of the bacteriophage as a biocontrol agent.

Main Methods:

  • Isolation and purification of bacteriophage PM1.
  • Electron microscopy for morphological analysis.
  • Whole-genome sequencing and bioinformatic analysis of predicted open reading frames (ORFs).

Main Results:

  • Bacteriophage PM1, belonging to the Myoviridae family, was isolated.
  • The phage possesses a 55,098-bp genome with a 2,665-bp terminal repeat and 44.9% GC-content.
  • PM1 encodes an RNA polymerase, indicative of T7-like phages, and lacks integrase/repressor genes, confirming its virulent nature.

Conclusions:

  • PM1 is a novel virulent bacteriophage with potential for controlling Pectobacterium carotovorum subsp. carotovorum.
  • Genomic analysis reveals characteristics shared with other T7-like phages.
  • Further studies are warranted to explore PM1's efficacy in biocontrol strategies.

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