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Laboratory control of immunocytochemistry
1Department of Haematology, Northern General Hospital, Sheffield, U.K.
European Journal of Haematology
|January 1, 1989
Summary
External quality assurance for immunocytochemistry revealed initial variations in results for monoclonal antibody techniques like alkaline phosphatase anti-alkaline phosphatase (APAAP) staining. Improvements were observed over the year, highlighting key variables in the technique.
Area of Science:
- Hematology
- Immunocytochemistry
- Molecular Diagnostics
Background:
- Monoclonal antibody technology is integral to hematology for analyzing and phenotyping malignancies.
- Immunocytochemical methods, especially the alkaline phosphatase anti-alkaline phosphatase (APAAP) technique, are gaining widespread use.
Purpose of the Study:
- To report the findings from the first year of an external quality assurance program for immunocytochemistry.
- To identify and highlight variables affecting the consistency of immunocytochemical techniques.
Main Methods:
- Implementation of an external quality assurance scheme for immunocytochemistry.
- Analysis of results from participating hematology departments using monoclonal antibody techniques.
Main Results:
- Initial analysis showed significant variability in reported results among laboratories.
- A noticeable improvement in result concordance was achieved over the year of the quality assurance scheme.
Conclusions:
- External quality assurance is crucial for standardizing immunocytochemistry in hematology.
- Variability in techniques, such as APAAP staining, can be addressed through quality improvement initiatives.