Leukaemic cell growth and aggregation in vitro

Experiments were performed with two lymphoblastic mouse leukaemia, L1210 VA II cell population growing in vitro for 90 and 400 days. These cells were cultured for 9 days in Eagle's solution without exchanging the medium, and each day a sample was taken for determination of its density, viability of cells and cell diameter. In the cells of the 400 day old population the presence and number of aggregates was checked. Lipid, nucleotide, nucleic acid, DNA and protein contents were determined in these cells. The present experiment in vitro was arranged to observe an aggregation of leukaemia cells during culture without change of the medium. The density and viability of cells changes of their metabolism in this system may stimulate the situation in vivo after dissemination of cells to a new place.