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Updated: May 2, 2026
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Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
Published on: August 10, 2017
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[Establishment of a quantitative detection method for Golgi protein73]
Summary
A new enzyme-linked immunosorbent assay (ELISA) was developed for quantifying Golgi protein 73 (GP73) in serum. This assay demonstrates high accuracy, sensitivity, and repeatability for detecting GP73 levels.
Area of Science:
- Biochemistry
- Immunology
Background:
- Golgi protein 73 (GP73) is a significant biomarker.
- Accurate quantification of GP73 in serum is crucial for diagnostic applications.
Purpose of the Study:
- To establish a reliable enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of GP73 in serum.
Main Methods:
- A sandwich ELISA was developed using a horseradish peroxidase-labeled monoclonal antibody against GP73.
- Optimization of reaction conditions and reagent concentrations was performed.
- The assay's performance was rigorously evaluated for linearity, sensitivity, specificity, and stability.
Main Results:
- The assay exhibited a linear range of 25-500 ng/ml with a detection limit of 18.5 ng/ml.
- Inter-assay and intra-assay coefficients of variation were below 10%.
- High recovery rates (92.6%-103.7%) and excellent stability were demonstrated.
Conclusions:
- The developed ELISA provides an accurate, sensitive, and repeatable method for serum GP73 quantification.
- This assay is suitable for clinical applications requiring precise measurement of GP73.
