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Related Concept Videos

RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
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The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
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Related Experiment Video

Updated: May 2, 2026

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
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A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

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[Recent progresses in RNA N6-methyladenosine research].

Yu-Li Li, Jun Yu, Shu-Hui Song

    Yi Chuan = Hereditas
    |March 21, 2014
    PubMed
    Summary

    N6-methyladenosine (m6A) is a key RNA modification. Recent advances in detection methods like immunoprecipitation sequencing (IP-seq) enable large-scale m6A studies, revealing its biological roles.

    Area of Science:

    • Molecular Biology
    • Epigenetics
    • RNA Biology

    Background:

    • N6-methyladenosine (m6A) is the most prevalent internal RNA modification in eukaryotes.
    • m6A is dynamically regulated by methyltransferases and demethylases, suggesting reversible biological roles.
    • Phenotypic changes observed upon altering m6A levels highlight its functional significance.

    Purpose of the Study:

    • To review recent advancements in m6A research.
    • To discuss the discovery, biosynthesis, distribution, and functions of m6A.
    • To compare current m6A detection methodologies, particularly IP-seq technologies.

    Main Methods:

    • Review of existing literature on m6A.
    • Comparison of three widely used immunoprecipitation sequencing (IP-seq) technologies for m6A detection.

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  • Analysis of m6A's role in various biological processes.

    • Main Results:

    • m6A is a reversible RNA modification with significant phenotypic consequences.
    • Next-generation sequencing combined with immunoprecipitation (IP) has enabled large-scale m6A detection.
    • Current IP-seq technologies vary in efficiency and application.

    Conclusions:

    • m6A plays crucial roles in RNA biology, but its mechanisms require further elucidation.
    • Advancements in detection technologies facilitate comprehensive m6A profiling.
    • Challenges remain in fully understanding m6A's biological functions and regulatory networks.