IDEXX SNAP beta-lactam ST validation for penicillin G detection.

Area of Science:

• Food safety and analytical chemistry
• Veterinary diagnostics

Background:

• Penicillin G is a common antibiotic used in dairy animal treatment.
• Residues of penicillin G in milk pose a public health concern and are regulated by maximum residue limits (MRLs).
• Accurate and rapid detection methods are crucial for ensuring milk quality and safety.

Purpose of the Study:

• To introduce and evaluate a new rapid test kit for the detection of penicillin G residues in various milk types.
• To confirm the test's optimized detection capability at or below the European Union MRL for penicillin G (4 parts per billion).

Main Methods:

• The SNAP Beta-Lactam ST Test Kit was utilized for penicillin G detection.
• The test was performed on commingled cow, goat, and sheep milk, as well as reconstituted whole fat powdered milk.
• No heat block or specialized reader was required for test execution.

Main Results:

• The SNAP Beta-Lactam ST Test Kit demonstrated robust performance in detecting penicillin G.
• The test achieved a detection capability at or below the EU MRL of 4 parts per billion.
• Assay time was approximately 7 minutes, with results readable visually or via specific readers.

Conclusions:

• The SNAP Beta-Lactam ST Test Kit offers an improved, rapid, and user-friendly method for detecting penicillin G in diverse milk matrices.
• The test meets stringent regulatory requirements for penicillin G residue levels in milk.
• This kit provides a valuable tool for ensuring the safety and quality of milk products.