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A simple fixation and embedding method for use in hybridization histochemistry on plant tissues
G I McFadden1, I Bonig, E C Cornish
1Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria, Australia.
The Histochemical Journal
|October 1, 1988
Abstract:
A simple method for fixing and embedding plant tissue for hybridization histochemistry has been developed. Material is fixed with paraformaldehyde-glutaraldehyde by the phase-partition technique and embedded in LR Gold. The method preserves nucleic acids in situ. Double-stranded 32P-labelled DNA probes hybridized to complementary RNAs in thin and semithin sections. Biotinylated probes detected with immunogold markers have been used to localize transcripts at the electron microscopic level.