Related Experiment Video
Updated: May 2, 2026
Normothermic Ex Vivo Pancreas Perfusion for the Preservation of Pancreas Allografts before Transplantation
Published on: July 27, 2022
Evaluation of Institut Georges Lopez-1 preservation solution in pig pancreas transplantation: a pilot study
Francisco A García-Gil1, Lorena Fuentes-Broto, Carlos D Albendea
11 Department of Surgery, University of Zaragoza, Zaragoza, Spain. 2 Department of Pharmacology and Physiology, University of Zaragoza, Zaragoza, Spain. 3 Gastroenterology and Hepatology Department, HCU Lozano Blesa, Zaragoza, Spain. 4 Experimental Hepatic Ischemia-Reperfusion Unit, Institute of Biomedical Research, Spanish National Research Council, Barcelona, Spain. 5 Department of Biochemistry and Molecular Cell Biology, University of Zaragoza, Zaragoza, Spain. 6 Department of Human Anatomy and Histology, University of Zaragoza, Zaragoza, Spain. 7 Department of Pathology, HCU Lozano Blesa, Zaragoza, Spain. 8 Department of Surgery, ICMDM, Hospital Clinic, University of Barcelona, Barcelona, Spain. 9 Address correspondence to: Prof. Francisco A. García-Gil, M.D., Ph.D., Department of Surgery, University of Zaragoza, Domingo Miral, s/n, 50009, Zaragoza, Spain.
Background:
Institut Georges Lopez-1 preservation solution (IGL-1) is an emerging extracellular-type electrolyte solution, low in viscosity, containing polyethylene glycol 35 as a colloid. Although IGL-1 has shown beneficial outcomes in kidney and liver preservation, this pilot study is the first to evaluate the efficacy of IGL-1 in pancreas transplantation (PT) compared with the University of Wisconsin solution (UW).
Methods:
Sixteen Landrace pigs underwent allogeneic PT with 16 hr of cold ischemia. Grafts were preserved with IGL-1 (n=8) or UW (n=8). No immunosuppression was administered. We analyzed graft function, the acute-phase response, and oxidative stress in the pancreatic graft monitoring membrane fluidity and lipid peroxidation.
Results:
All eight grafts with IGL-1, but only six with UW, were functioning. Graft failures with UW resulted from graft thrombosis. There were no differences between the two solutions in the number of normoglycemic days (IGL-1: 11.5 ± 6.2 versus UW: 8.5 ± 4.4 days, P=0.1357), nor in lipid peroxidation during 16-hr cold ischemia (P=0.672), or reperfusion (P=0.185), but IGL-1 prevented changes in membrane fluidity after reperfusion when compared with UW (P=0.026).
Conclusion:
IGL-1 offered the same degree of safety and effectiveness as UW in our model of pig PT with 16 hr of cold ischemia.
