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Updated: Jul 30, 2026

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus
Published on: June 24, 2012
Inhibition of RNase H activity and viral replication by single mutations in the 3' region of Moloney murine leukemia
R Repaske1, J W Hartley, M F Kavlick
1Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Abstract:
Selected conserved amino acids in the putative RNase H domain of reverse transcriptase (RT) were modified in a molecularly cloned infectious provirus and in a Moloney murine leukemia virus RT expression vector by site-directed mutagenesis. Substitution of either of two conserved aspartic acid residues in proviral DNA prevented production of infectious particles in transfected NIH 3T3 cells, and the same modifications depressed RT-associated RNase H activity by more than 25-fold with little or no effect on polymerase activity.
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