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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

3.1K
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Immunoprecipitation01:20

Immunoprecipitation

5.6K
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
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Types Of Column Chromatography01:29

Types Of Column Chromatography

10.8K
The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
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Affinity and Avidity01:41

Affinity and Avidity

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Overview
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Related Experiment Video

Updated: May 2, 2026

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
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Affinity chromatography for antibody purification.

Sushrut Arora1, B Vijayalakshmi Ayyar, Richard O'Kennedy

  • 1National Centre for Sensor Research, Biomedical Diagnostics Institute, Dublin City University, Dublin, Ireland.

Methods in Molecular Biology (Clifton, N.J.)
|March 21, 2014
PubMed
Summary

Purifying antibodies is essential for research and diagnostics. Affinity chromatography offers a highly selective, rapid, and simple method for obtaining pure monoclonal, polyclonal, and recombinant antibodies.

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Area of Science:

  • Biochemistry
  • Immunology
  • Biotechnology

Background:

  • Purified antibodies are critical reagents for diverse biological and diagnostic applications.
  • Selecting appropriate antibody purification strategies is paramount for experimental success.
  • While various purification methods exist, affinity chromatography is the most widely adopted technique.

Purpose of the Study:

  • To outline fundamental techniques for antibody purification.
  • To highlight the advantages of affinity chromatography in antibody purification.
  • To provide guidance on achieving high purity and yield for different antibody types.

Main Methods:

  • Focuses on affinity chromatography as the primary purification strategy.
  • Explains the principles of specific biological interactions utilized in affinity chromatography.
  • Describes the application of these techniques to monoclonal, polyclonal, and recombinant antibodies.

Main Results:

  • Affinity chromatography provides high selectivity and minimal procedural steps.
  • This method ensures simplicity and speed in antibody purification.
  • Effective protocols require careful planning and validation for optimal outcomes.

Conclusions:

  • Affinity chromatography is a powerful and versatile technique for antibody purification.
  • The described methods enable the isolation of high-purity antibodies.
  • Successful implementation leads to reliable antibody preparations for various applications.