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Related Experiment Video

Updated: May 2, 2026

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Modulating endothelial barrier function by targeting vimentin phosphorylation.

Tiegang Liu1, Maher M Ghamloush, Ali Aldawood

  • 1Pulmonary & Critical Care Division, Department of Medicine/Tupper Research Institute, Tufts Medical Center, Tufts University School of Medicine, Boston, Massachusetts.

Journal of Cellular Physiology
|March 21, 2014
PubMed
Summary
This summary is machine-generated.

Vimentin phosphorylation regulates endothelial barrier integrity. Blocking vimentin phosphorylation protects against injury and may offer therapeutic strategies for conditions like acute respiratory distress syndrome (ARDS).

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Area of Science:

  • Cell Biology
  • Biochemistry
  • Physiology

Background:

  • Vimentin, a key intermediate filament protein, is crucial for vascular endothelial cell function.
  • Endothelial cells form a barrier tissue, maintaining integrity against shear and contractile forces.
  • Vimentin's role in regulating endothelial permeability is linked to its phosphorylation state.

Purpose of the Study:

  • To investigate the role of vimentin phosphorylation in maintaining endothelial barrier function.
  • To determine the effects of Withaferin A (WFA) on vimentin and endothelial barrier properties.
  • To explore therapeutic potential of targeting vimentin phosphorylation for barrier protection.

Main Methods:

  • Vimentin phosphorylation analysis.
  • Endothelial cell culture and barrier function assays.
  • Overexpression of non-phosphorylatable vimentin mutants.
  • Treatment with Withaferin A (WFA) and LPS endotoxin.

Main Results:

  • Withaferin A disrupts endothelial barrier function by altering vimentin distribution and properties, linked to increased vimentin phosphorylation.
  • Overexpression of a non-phosphorylatable vimentin mutant prevents WFA-induced barrier disruption.
  • Blocking vimentin phosphorylation protects the endothelial barrier against LPS-induced injury.
  • Potential transmissible effects of vimentin phosphorylation modulation across endothelial cells observed.

Conclusions:

  • Vimentin phosphorylation is a critical mechanism for regulating endothelial barrier integrity.
  • Targeting vimentin phosphorylation presents a potential therapeutic strategy for pulmonary edema and acute respiratory distress syndrome (ARDS).
  • Further research into vimentin's role could lead to novel treatments for endothelial barrier dysfunction.