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Hormonal changes in exfoliated normal buccal mucosal cells.

Preethy Mary Donald1, Renjith George1, G Sriram2

  • 1Department of Oral Medicine, Oral Diagnosis and Oral Pathology, Faculty of Dentistry, Melaka-Manipal Medical College, Melaka, Malaysia.

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|March 21, 2014
PubMed
Summary

Buccal smears show age-related changes in nuclear diameter (ND) and cell diameter (CD). These cellular metrics vary significantly across different female age groups, indicating alterations with aging.

Keywords:
Cell diametercytomorphometric analysisexfoliative cytologynormal buccal mucosal cellsnucleus diameternucleus-cytoplasmic ratio

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Area of Science:

  • Cytology
  • Gerontology
  • Biomarkers

Background:

  • Buccal smears are routinely used for cytological analysis.
  • Understanding cellular changes with age is crucial for baseline health assessments.

Purpose of the Study:

  • To evaluate nuclear diameter (ND), cell diameter (CD), and nuclear-cytoplasmic ratio (N:C) in normal female buccal smears.
  • To assess variations in these parameters with advancing age.

Main Methods:

  • Collected buccal smears from 400 healthy female subjects.
  • Utilized Papanicolaou staining and measured ND and CD with micrometry.
  • Analyzed age-related differences using ANOVA and Tukey's HSD tests.

Main Results:

  • Observed an increase in ND and CD from childhood to adulthood (5-35 years).
  • Noted a decline in ND and CD in older age groups (45-60 years).
  • Reported variations in N:C ratio and exfoliation patterns across age groups.

Conclusions:

  • Buccal smear parameters exhibit age-related alterations in females.
  • These findings establish a baseline for age-associated cytomorphological changes.