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Human Lung Mast Cell Products Regulate Airway Smooth Muscle CXCL10 Levels
H Alkhouri1, V Cha1, K Tong1
1Faculty of Pharmacy, The University of Sydney, Sydney, NSW 2006, Australia.
Journal of Allergy
|March 21, 2014
Summary
Mast cells regulate airway smooth muscle CXCL10 production. Mast cell tryptase and early supernatants reduce CXCL10, while later supernatants increase it, suggesting a role in asthma.
Area of Science:
- Immunology
- Respiratory Medicine
- Cell Biology
Background:
- Asthma involves airway smooth muscle (ASM) producing CXCL10, attracting immune cells.
- The role of mast cells (MC) in regulating ASM CXCL10 production is not fully understood.
Purpose of the Study:
- To investigate how mast cell products affect ASM cell CXCL10 production.
- To determine if mast cell mediators influence CXCL10 at the protein or mRNA level.
Main Methods:
- ASM cells were stimulated with cytokines and treated with histamine, tryptase, or mast cell supernatants.
- CXCL10 production was measured using ELISA and real-time PCR.
- Mast cells were isolated, activated, and their supernatants collected and applied to ASM cells.
Main Results:
- Histamine reduced CXCL10 protein but not mRNA, independent of H1/H2 receptors.
- Tryptase and early mast cell supernatants decreased cytokine-induced CXCL10 production.
- Late mast cell supernatants contained TNF-α and enhanced IFN-γ-induced CXCL10 production.
Conclusions:
- Mast cells can modulate ASM cell CXCL10 production and degradation.
- Mast cell mediators play a complex role in regulating airway inflammation in asthma.
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