Determination of imidocarb in animal biosamples using simple high-performance liquid chromatography with diode-array...

Area of Science:

• Analytical Chemistry
• Food Safety Analysis
• Pharmacology

Background:

• Imidocarb is a drug used in veterinary medicine.
• Accurate detection of imidocarb residues in food products is crucial for public health.
• Existing analytical methods may lack sensitivity or efficiency.

Purpose of the Study:

• To develop and validate a sensitive analytical method for imidocarb detection.
• To establish limits of quantification (LOQs) in beef and milk matrices.
• To assess the precision and accuracy of the developed method.

Main Methods:

• High-performance liquid chromatography (HPLC) with diode-array detection (DAD).
• Reversed-phase column chromatography with a specific mobile phase composition.
• Solid-phase extraction (SPE) for sample preparation.
• Detection wavelength set at 260 nm.

Main Results:

• The method demonstrated good linearity (r≥0.998).
• LOQs were 0.15 mg/kg for beef and 0.025 mg/kg for milk.
• High precision (3.2-6.9%) and accuracy (80.1-89.5% recovery) were achieved.

Conclusions:

• A validated SPE-HPLC-DAD method for imidocarb analysis in beef and milk was successfully developed.
• The method is suitable for routine analysis due to its acceptable precision and recovery.
• This contributes to enhanced food safety monitoring for veterinary drug residues.