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A modified Brown and Hopps stain for identification of gram-positive and gram-negative microorganisms in glycol
D A Schwartz1, R Munger, B Lazzaro
1Department of Pathology and Laboratory Medicine, Hahnemann University School of Medicine, Philadelphia.
Abstract:
Plastic embedding is a recently developed technique that has been shown to be superior to conventional paraffin embedding in the histopathologic identification of microorganisms in tissue specimens. This report describes a modification of the Brown and Hopps stain for the differentiation of gram-positive and gram-negative microorganisms in 3-micron-thick plastic sections. This technique is easily performed and has a rapid turnaround time. This staining method permits a more precise histopathologic diagnosis of infectious agents than is possible in paraffin-embedded tissues.
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