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Keratin patterns in prostatic hyperplasia and adenocarcinoma
H Svanholm1, B Nielsen, H Starklint
1Department of Pathology, University Hospital of Odense, Denmark.
APMIS. Supplementum
|January 1, 1988
Summary
Keratin patterns differ between benign prostatic hyperplasia and adenocarcinoma. Immunohistochemical staining reveals distinct keratin profiles in basal and luminal cells, influenced by tissue fixation methods and antibody choice, aiding in cancer diagnosis.
Area of Science:
- Urology
- Pathology
- Immunohistochemistry
Background:
- Benign prostatic hyperplasia (BPH) and prostatic adenocarcinoma share some cellular origins, necessitating precise diagnostic markers.
- Keratin expression is a key factor in differentiating epithelial cell types and states.
Purpose of the Study:
- To investigate and compare keratin expression patterns in benign prostatic hyperplasia (BPH) and prostatic adenocarcinoma.
- To evaluate the impact of tissue fixation (frozen vs. formalin-fixed) and antibody selection on keratin staining results.
- To assess the diagnostic utility of specific antibodies, like Z622, in distinguishing neoplastic lesions.
Main Methods:
- Analysis of keratin patterns in BPH and adenocarcinoma tissues using both frozen and formalin-fixed samples.
- Application of five distinct commercially available antibodies (PKE, MCA 144, CAM 5.2, RCK 102, Z622) for immunohistochemical staining.
- Detailed comparison of staining results between basal and luminal cells in benign and malignant tissues.
Main Results:
- Distinct keratin staining patterns were observed in basal and luminal cells of benign prostatic tissues, varying with fixation method.
- Formalin-fixed tissues showed exclusive basal cell staining with Z622, while luminal cells in adenocarcinomas predominantly expressed MCA 144 and CAM 5.2.
- The antibody Z622 demonstrated potential in differentiating invasive carcinoma from intraluminal neoplastic lesions, especially in cribriform patterns.
Conclusions:
- Keratin expression profiles in benign and malignant prostate epithelium are influenced by immunohistochemical procedures.
- Antibody selection and tissue fixation are critical variables affecting keratin pattern analysis.
- Specific antibodies, such as Z622, may serve as valuable tools in the pathological diagnosis of prostate cancer, particularly for challenging cases.