Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genetic Screens02:46

Genetic Screens

4.6K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
4.6K
Block Diagram Reduction01:22

Block Diagram Reduction

727
The process of deriving the transfer function of a control system often involves reducing its block diagram to a single block. This simplification can be achieved through a series of strategic operations, including relocating branch points and comparators. These operations preserve the overall function of the system while allowing for easier manipulation and combination of blocks.
The first step in this process is the identification and relocation of a branch point. A branch point, where a...
727
Epistasis Analysis01:09

Epistasis Analysis

4.9K
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
4.9K
Circuit Terminology01:14

Circuit Terminology

3.1K
An electrical network is a system composed of interconnected elements, such as resistors, capacitors, inductors, and voltage or current sources. Unlike a circuit, an electrical network does not necessarily form a closed path. In other words, while all circuits can be considered networks due to their interconnected nature, not every network qualifies as a circuit.
A circuit, on the other hand, is also an interconnected system of electrical elements but must contain one or more closed paths.
3.1K
Signal Flow Graphs01:18

Signal Flow Graphs

843
Signal-flow graphs offer a streamlined and intuitive approach to representing control systems, providing an alternative to traditional block diagrams. These graphs use branches to symbolize systems and nodes to represent signals, effectively illustrating the relationships and interactions within the system.
In a signal-flow graph, branches denote the system's transfer functions, while nodes represent the signals. The direction of signal flow is indicated by arrows, with the corresponding...
843
Synthetic Biology02:55

Synthetic Biology

4.4K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Developing a standard definition for sequences of concern.

Frontiers in bioengineering and biotechnology·2026
Same author

From FAIR to CURE: guidelines for computational models of biological systems.

NPJ systems biology and applications·2026
Same author

A Framework for a Standard-Enabled FAIR Data Management Workflow for Synthetic Biology.

ACS synthetic biology·2026
Same author

A Decade of SBOL Visual: Growing Adoption of a Diagram Standard for Engineering Biology.

ACS synthetic biology·2025
Same author

IWBDA 2023: Tools and Frameworks Take Flight.

ACS synthetic biology·2025
Same author

Improved provenance tracking by documenting plasmids within their own sequence.

Trends in biotechnology·2025

Related Experiment Video

Updated: May 2, 2026

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.9K

Directed acyclic graph-based technology mapping of genetic circuit models.

Nicholas Roehner1, Chris J Myers

  • 1Department of Bioengineering, University of Utah , Salt Lake City 84112, United States.

ACS Synthetic Biology
|March 22, 2014
PubMed
Summary

Synthetic biology advances require genetic design automation (GDA) tools. We developed a novel algorithm using electronic circuit design principles to automatically select DNA parts for genetic circuits, optimizing design processes.

More Related Videos

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

46.1K
Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

14.2K

Related Experiment Videos

Last Updated: May 2, 2026

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.9K
Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

46.1K
Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

14.2K

Area of Science:

  • Synthetic Biology
  • Computational Biology
  • Bioengineering

Background:

  • Synthetic biology relies on standardized DNA components and abstraction for complex genetic circuit design.
  • Existing genetic design automation (GDA) tools lack sophisticated methods for automatically selecting components from libraries.
  • The maturation of engineering principles necessitates advanced computational tools for efficient genetic circuit construction.

Purpose of the Study:

  • To develop and implement a genetic technology mapping algorithm for GDA tools.
  • To adapt established electronic circuit design mapping techniques for synthetic biology applications.
  • To enable automated selection of standardized DNA components based on behavioral specifications.

Main Methods:

  • Developed a genetic technology mapping algorithm based on directed acyclic graph (DAG) techniques.
  • Integrated the algorithm into the iBioSim genetic design automation tool.
  • Utilized a cost function, adapted from electronic circuit design, to guide the search for optimal component selection.
  • Employed standards for describing genetic function and structure in design specifications and component libraries.

Main Results:

  • The algorithm successfully mapped specifications for three distinct genetic circuits.
  • Performance was evaluated against exhaustive and greedy search variants using randomly generated component libraries of increasing size.
  • The developed algorithm demonstrated effectiveness in finding optimal and near-optimal solutions for genetic circuit design.

Conclusions:

  • The novel genetic technology mapping algorithm enhances the capabilities of GDA tools in synthetic biology.
  • Adapting electronic circuit design methodologies provides a powerful framework for automating genetic circuit construction.
  • This work represents a significant step towards efficient and standardized design of complex genetic systems.