Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Correlation of near-field optical microscopy and tip-assisted photoluminescence.

Journal of microscopy·2025

Same author

Real-space observation of ultraconfined in-plane anisotropic acoustic terahertz plasmon polaritons.

Nature materials·2023

Same author

Time-resolved relaxation and fragmentation of polycyclic aromatic hydrocarbons investigated in the ultrafast XUV-IR regime.

Nature communications·2021

Same author

Hyperspectral Nanoimaging of van der Waals Polaritonic Crystals.

Nano letters·2021

Same author

Planar refraction and lensing of highly confined polaritons in anisotropic media.

Nature communications·2021

Same author

Structures and internal dynamics of diphenylether and its aggregates with water.

Physical chemistry chemical physics : PCCP·2020

Search research articles

Related Experiment Video

Updated: May 2, 2026

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Published on: March 31, 2022

Synthetic optical holography for rapid nanoimaging.

M Schnell¹, P S Carney², R Hillenbrand³

¹CIC nanoGUNE Consolider, 20018 Donostia-San Sebastian, Spain.

Nature Communications

|March 22, 2014

Summary

Synthetic optical holography (SOH) enables fast, quantitative phase imaging in scanning microscopy. This technique accelerates nanoscale imaging of materials like graphene, overcoming previous limitations.

More Related Videos

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Related Experiment Videos

Last Updated: May 2, 2026

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging

Published on: March 31, 2022

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Area of Science:

Optics and Photonics
Materials Science
Microscopy Techniques

Background:

Holography is crucial for phase imaging across various microscopy types.
Serial image acquisition in scanning optical microscopy hinders traditional holography.
Quantitative phase imaging is essential for nanoscale material characterization.

Purpose of the Study:

Introduce synthetic optical holography (SOH) for scanning optical microscopy.
Enable fast, quantitative phase-resolved imaging with technical simplicity.
Demonstrate simultaneous visible and infrared frequency operation.

Main Methods:

Developed synthetic optical holography (SOH) for scanning microscopy.
Integrated SOH into a scattering-type scanning near-field optical microscope (s-SNOM).
Utilized a single reference arm for dual-frequency operation.

Main Results:

Achieved unprecedented speed in quantitative phase-resolved near-field imaging.
Successfully screened grain boundaries in CVD-grown graphene.
Acquired 65-kilopixel images in 26 seconds and 2.3-megapixel images in 13 minutes.

Conclusions:

SOH overcomes limitations of traditional holography in scanning microscopy.
The technique offers rapid, non-invasive nanoscale imaging capabilities.
SOH has potential applications in other scanning imaging techniques like confocal microscopy.