Related Experiment Video

Updated: May 2, 2026

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.5K

A screen identifies the oncogenic micro-RNA miR-378a-5p as a negative regulator of oncogene-induced senescence

Susanne Marije Kooistra1, Lise Christine Rudkjær Nørgaard1, Michael James Lees1

  • 1Biotech Research and Innovation Centre, University of Copenhagen, Copenhagen, Denmark; Centre for Epigenetics, University of Copenhagen, Copenhagen, Denmark.

Plos One
|March 22, 2014
PubMed

Insights

MicroRNAs can influence cancer development. Researchers found that miR-378a-5p delays oncogene-induced senescence (OIS), a process that normally prevents tumor formation, suggesting a pro-tumor role for this microRNA.

Area of Science:

  • Cellular senescence
  • Oncogenesis
  • MicroRNA biology

Background:

  • Oncogene-induced senescence (OIS) acts as a tumor suppressor mechanism.
  • Identifying factors that regulate OIS is crucial for understanding cancer development.

Purpose of the Study:

  • To discover novel microRNAs that can modulate OIS.
  • To investigate the role of miR-378a-5p in OIS and its potential impact on tumorigenesis.

Main Methods:

  • Screening for microRNAs affecting OIS in human diploid fibroblasts.
  • Assessing senescence markers (e.g., p16INK4A, senescence-associated β-galactosidase) upon ectopic miR-378a-5p expression.
  • Analyzing miR-378a-5p targets to elucidate its mechanism of action.

Main Results:

  • miR-378a-5p was identified as a microRNA capable of inhibiting OIS.
  • Ectopic miR-378a-5p expression reduced senescence markers and maintained proliferative capacity in the presence of oncogenic BRAF.
  • Several miR-378a-5p targets were identified, providing mechanistic insights into OIS delay.

Conclusions:

  • miR-378a-5p delays oncogene-induced senescence in human diploid fibroblasts.
  • This delay in OIS by miR-378a-5p may contribute to tumor formation, aligning with its known pro-oncogenic function.

More Related Videos

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR08:30

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

24.0K
An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.1K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.1K
MicroRNAs01:22

MicroRNAs

9.8K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.6K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
32.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.0K