Polymer-tethered lipid multi-bilayers: a biomembrane-mimicking cell substrate to probe cellular mechano-sensing

Cells tiptoe through their environment forming highly localized and dynamic focal contacts. Experiments on polymeric gels of adjustable elasticity have shown that cells probe the viscoelasticity of their environment through an adaptive process of focal contact assembly/disassembly that critically affects cell adhesion, morphology, and motility. However, the specific mechanisms of this process have not yet been fully revealed. Here we report, for the first time, that fibroblast adhesion, morphology, and migration can also be controlled by altering the number of bilayers in a stack of multiple polymer-tethered lipid bilayers stabilized via maleimide-sulfhydral coupling chemistry. The observed changes in cell morphology, migration, and cytoskeletal organization in response to bilayer stacking correspond well with those previously observed on polymeric substrates of different polymer crosslinking density suggesting that variations in bilayer stacking are associated with changes in substrate viscoelasticity. This is in conceptual agreement with the existing knowledge about the structural, dynamic, and mechanical properties of polymer-lipid composite materials. Several distinct features, such as the lateral mobility of individual cell linkers and the immobilization of linker clusters, make the described substrates highly attractive tools for the study of dynamic, mechano-regulated cell linkages and cellular mechano-sensing.