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Updated: May 2, 2026

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Published on: November 28, 2015

Label-free cell-based dynamic mass redistribution assays.

Hannah J Gitschier¹, Audrey B Bergeron¹, David H Randle¹

¹Corning Life Sciences, Kennebunk, Maine.

Current Protocols in Chemical Biology

|March 22, 2014

Summary

This summary is machine-generated.

Label-free cell assays using dynamic mass redistribution (DMR) provide phenotypic profiles for drug discovery. This method analyzes compound pharmacology in various cell types by examining kinetic traces from receptor stimulation.

Keywords:

cell surface receptors drug discovery dynamic mass redistribution label-free optical biosensor phenotypic assays primary cells signal transduction pathways

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Last Updated: May 2, 2026

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Published on: August 6, 2013

Area of Science:

Biochemistry
Cell Biology
Pharmacology

Background:

Label-free cell-based assays are crucial for drug discovery and compound profiling.
Endogenously expressed receptors in diverse cell types, including primary and stem cells, are key targets.
Dynamic mass redistribution (DMR) offers a label-free method to assess cellular responses.

Purpose of the Study:

To provide protocols for obtaining DMR response profiles in both adherent and suspension cells.
To demonstrate the use of tool compounds for delineating signaling pathway biology.
To leverage information-rich kinetic traces for evaluating compound pharmacology.

Main Methods:

Utilizing label-free cell-based assays to measure dynamic mass redistribution (DMR).
Implementing protocols for DMR analysis in adherent and suspension cell formats.
Employing known tool compounds to stimulate receptors and record kinetic traces.

Main Results:

DMR responses provide phenotypic activity profiles for evaluating compound pharmacology.
Kinetic traces offer detailed insights into cellular signaling pathways.
The methodology is applicable to a wide range of cell types.

Conclusions:

Label-free DMR assays are a powerful tool for drug discovery and compound profiling.
The described protocols facilitate the analysis of receptor pharmacology in various cell models.
DMR kinetic data enables comprehensive understanding of signaling pathway biology.