Alarms and parameters generated by hematology analyzer: new tools to predict and quantify circulating Sezary cells

Area of Science:

• Hematology
• Clinical Cytology
• Oncology

Background:

• Cytological review of circulating neoplastic cells is crucial for diagnosis and prognosis.
• Manual or automated microscopic analysis requires expert cytologists.
• Hematology analyzer parameters can alert operators to potential abnormalities.

Purpose of the Study:

• To evaluate the utility of hematology analyzer parameters in detecting Sezary syndrome.
• To correlate specific analyzer parameters with Sezary cell characteristics and prognosis.

Main Methods:

• Blood samples from Sezary syndrome patients (n=9) were analyzed using a Sysmex XN-1000 analyzer.
• Control groups included patients with benign/tumoral skin lesions (n=47) and chronic lymphoproliferative B-cell diseases (n=51).

Main Results:

• Structural lymphoid parameters (LyX, LyZ) and the Blast/Abn Lympho alarm were significantly higher in Sezary cases versus controls.
• LyX values correlated with the count of circulating Sezary cells.
• LyZ values correlated with the presence of large Sezary cells, both identified as prognostic factors.

Conclusions:

• The combination of Blast/Abn Lympho alarm and structural parameters (Ly-X/Ly-Z/Ly-Y) can help establish rules for blood slide review.
• This approach shows promise for screening circulating Sezary cells, particularly in B-cell diseases.