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Prevalence and molecular characterization of human metapneumovirus in influenza a negative sample in Thailand

Navin Horthongkham1, Niracha Athipanyasilp, Rujipas Sirijatuphat

  • 1Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.

Journal of Clinical Laboratory Analysis
|March 22, 2014
PubMed
Abstract

Insights

Human metapneumovirus (hMPV) was detected in 6.3% of respiratory infections in Thailand. Subgenotype B1 was the most prevalent strain, particularly in young children.

Area of Science:

  • Virology
  • Epidemiology
  • Public Health

Background:

  • Human metapneumovirus (hMPV) is a significant cause of respiratory tract infections, often presenting as influenza-like illness.
  • The epidemiological role of hMPV across all age groups in Thailand remained largely uncharacterized prior to this study.

Purpose of the Study:

  • To determine the prevalence of hMPV infections in all age groups within Thailand.
  • To identify the predominant hMPV genotypes circulating in the country during 2011.

Main Methods:

  • Analysis of 1,184 nasopharyngeal washes from hospitalized patients.
  • Detection of hMPV using real-time polymerase chain reaction (PCR).
  • Sequencing of the F gene for phylogenetic analysis and genotyping.

Main Results:

  • An overall hMPV prevalence of 6.3% was observed across all age groups.
  • The highest prevalence was noted in children under 2 years of age.
  • Subgenotype B1 accounted for 96.8% of detected hMPV strains, with minor occurrences of A2a and A2b sublineages.

Conclusions:

  • The study identified hMPV subgenotype B1 as the dominant strain circulating in Thailand in 2011.
  • This finding contributes to understanding hMPV epidemiology in Southeast Asia.
Keywords:
Thailandhuman metapnuemovirusinfluenza-like illnessprevalence

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