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Updated: May 2, 2026
Long-term Potentiation of Perforant Pathway-dentate Gyrus Synapse in Freely Behaving Mice
Published on: November 29, 2013
Modulation of the perforant path-evoked potential in dentate gyrus as a function of intrahippocampal β-adrenoceptor
Rebecca L Lethbridge1, Susan G Walling1, Carolyn W Harley1
1Behavioral Neuroscience, Psychology Department, Memorial University of Newfoundland St. John's, NL, A1B 3X9, Canada.
Background:
β-adrenoceptor activation in the hippocampus is sufficient to induce heterosynaptic long-term potentiation of perforant path input to the dentate gyrus. However, in vitro and in vivo studies suggest the plasticity effects of β-adrenoceptor activation may vary depending on the level of receptor activation.
Methods:
The present experiments use an in vivo model concurrently infusing differing concentrations of the β-adrenoceptor agonist, isoproterenol (ISO; 0, 0.1, 1, 10, and 100 μmol/L in aCSF; 1 μL over 12.5 min) in the dentate gyrus, while monitoring changes in the perforant path-evoked potential at the same site.
Results:
Long-term depression (LTD) of fEPSP slope was elicited by 0.1 μmol/L ISO. Higher doses did not alter fEPSP slope. Maximal long-term potentiation of the perforant path-evoked population spike (183% >3 h) occurred at 10 μmol/L ISO. Transient depression of spike amplitude occurred at 0.1 μmol/L ISO.
Conclusions:
These data demonstrate concentration-dependent effects of β-adrenoceptor activation on the perforant path-evoked potential. Long-term depression and long-term potentiation of perforant path-evoked responses are variably elicited as a function of the degree of receptor activation.
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