Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human immunodeficiency viruses: neutralization and receptors.

R A Weiss1, P R Clapham, M O McClure

  • 1Institute of Cancer Research, Chester Beatty Laboratories, London, U.K.

Journal of Acquired Immune Deficiency Syndromes
|January 1, 1988
PubMed
Summary

Neutralizing antibodies target HIV envelope glycoproteins gp120 and gp41. The CD4 receptor is crucial for HIV entry into T-lymphocytes and monocytes, but not essential for glial or muscle cell infection.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of Gemcitabine based chemotherapy on the immunogenicity of pancreatic tumour cells and T-cells.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2020
Same author

Gemcitabine alters the proteasome composition and immunopeptidome of tumour cells.

Oncoimmunology·2018
Same author

TNF superfamily members promote hepatitis C virus entry via an NF-κB and myosin light chain kinase dependent pathway.

The Journal of general virology·2016
Same author

Lentiviral hepatitis B pseudotype entry requires sodium taurocholate co-transporting polypeptide and additional hepatocyte-specific factors.

The Journal of general virology·2015
Same author

Dynamics of immunoglobulin sequence diversity in HIV-1 infected individuals.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2015
Same author

The T4 molecule: function and structure.

Immunology today·2014

Area of Science:

  • Virology
  • Immunology
  • Molecular Biology

Background:

  • Human immunodeficiency virus (HIV) envelope glycoproteins, gp120 and gp41, possess epitopes targeted by neutralizing antibodies.
  • Group-specific neutralization antigens common to most HIV-1 isolates exist, with some HIV-2 antisera cross-neutralizing HIV-1.
  • Neutralization epitopes on HIV-1 gp41 (group-specific) and gp120 (type-specific) have been identified and mapped.

Purpose of the Study:

  • To investigate the role of CD4 as a receptor for HIV.
  • To characterize the binding interaction between HIV gp120 and the CD4 receptor.
  • To assess the necessity of CD4 for HIV infection in different cell types.

Main Methods:

  • Analysis of human sera from HIV-infected individuals.

Related Experiment Videos

  • In vitro selection of neutralization escape mutants using monoclonal antibodies (mAbs).
  • Binding assays using CD4 antigen and HIV-1 gp120.
  • Experiments with anti-idiotypic sera against CD4 mAbs and soluble recombinant CD4 (sCD4).
  • Assessment of HIV plating on glial and muscle tumor cells with and without anti-CD4 mAbs or sCD4.

    • Main Results:

    • The CD4 antigen binds HIV-1 gp120 with high affinity, serving as the receptor for HIV-1, HIV-2, and SIV on T-lymphocytes and monocytes.
    • HIV internalization occurs via a pH-independent process following CD4 binding, with the N-terminal V domain of CD4 being the primary binding site for gp120.
    • Anti-idiotypic sera to CD4 mAbs and sCD4 demonstrated neutralization capabilities against various HIV strains, while neither inhibited HIV plating on glial or muscle cells.

    Conclusions:

    • CD4 is essential for HIV entry into T-lymphocytes and monocytes, mediating high-affinity binding and subsequent internalization.
    • The CD4-gp120 interaction is a key target for therapeutic neutralization strategies.
    • CD4 is not indispensable for HIV infection of certain non-lymphoid cell types, such as glial and muscle cells.