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Updated: May 1, 2026

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

A versatile method for encapsulating large-sized DNA into small-sized bioreducible nanocapsules.

Long-Hai Wang¹, Sheng-Gang Ding, Jun-Jie Yan

¹CAS Key Lab of Soft Matter Chemistry, Department of Polymer Science and Engineering, University of Science and Technology of China , Hefei 230026, Anhui, People's Republic of China.

The Journal of Physical Chemistry. B

|March 25, 2014

Summary

Researchers developed a novel method to encapsulate large plasmid DNA into small bioreducible nanocapsules without condensing agents. This technique utilizes shearing force and temperature triggers for efficient DNA delivery systems.

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Area of Science:

Biotechnology
Materials Science
Nanotechnology

Background:

Encapsulating large plasmid DNA into small nanocapsules without condensing agents presents a significant challenge in gene delivery.
Existing methods often require harsh chemicals or complex procedures, limiting their applicability.

Purpose of the Study:

To develop a versatile and efficient method for encapsulating large-sized plasmid DNA into small-sized bioreducible nanocapsules.
To overcome the limitations of current DNA encapsulation techniques.

Main Methods:

Utilizing shearing force and surfactant to fold large plasmid DNA into small emulsion droplets containing bioreducible branched polymers.
Employing a temperature trigger to induce aggregation and cross-linking of polymers at the water/oil interface, forming a bioreducible shell.
Removing the surfactant to yield the final small-sized nanocapsule.

Main Results:

Successfully encapsulated large-sized plasmid DNA (approx. 1900 nm) into small-sized nanocapsules (approx. 110 nm).
Demonstrated the formation of a bioreducible shell around the nanodroplets.
Achieved encapsulation without the need for any condensing agents.

Conclusions:

The developed method offers a promising approach for creating small bioreducible nanocapsules for large plasmid DNA.
This technique has potential applications in gene therapy and other nucleic acid delivery systems.
The method is versatile and overcomes previous limitations in DNA encapsulation.