Real-time imaging of axonally transported subresolution organelles in vertebrate myelinated axons

A procedure is described for the real-time imaging of organelles of sub-resolution dimensions that undergo rapid axonal transport in myelinated axons. The procedure uses commercially available processors to enhance images that are obtained with a video microscope. Image detail is enhanced by spatial high-pass filtering. Small moving organelles are detected by subtracting a dynamically updated image of stationary cellular detail from one that contains the same stationary features plus images of the moving organelles.