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Updated: May 1, 2026
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
Threat of drug resistant Staphylococcus aureus to health in Nepal
Shamshul Ansari1, Hari Prasad Nepal, Rajendra Gautam
1Department of Microbiology, Chitwan Medical College, Bharatpur, Chitwan, Nepal. shamshulansari483@yahoo.com.
Background:
Staphylococcus aureus is the most commonly isolated organism from the different clinical samples in hospital. The emergence and dissemination of methicillin resistant Staphylococcus aureus (MRSA) and growing resistance to non-beta-lactam antibiotics is making treatment of infections due to this organism increasingly difficult.
Methods:
This study was conducted to determine the frequency of Staphylococcus aureus isolated from different clinical samples, rates of MRSA and full antibiotic susceptibility profiles. Clinical samples were cultured and Staphylococcus aureus was identified using standard microbiological methods recommended by the American Society for Microbiology (ASM). Methicillin resistance was confirmed using cefoxitin and oxacillin disks. Inducible clindamycin resistance was identified using D-zone test.
Results:
From the processed samples, 306 isolates of Staphylococcus aureus were recovered. All the isolates were susceptible to vancomycin and teicoplanin. Methicillin resistance was observed in 43.1% of isolates while inducible clindamycin resistance in 12.4% of the isolates.
Conclusions:
The results of our study reveals that rates of resistance to commonly prescribed antibiotics in Staphylococcus aureus clinical isolates is high. In particular, rate of methicillin resistance is alarming, prompting concern on the rational use of antibiotics and vigilant laboratory-based surveillance of resistance rates in Nepal.
Insights
High rates of methicillin-resistant Staphylococcus aureus (MRSA) were found in clinical samples. This highlights the urgent need for antibiotic stewardship and surveillance of Staphylococcus aureus resistance patterns.
Area of Science:
- Clinical microbiology
- Infectious diseases
- Antimicrobial resistance
Background:
- Staphylococcus aureus is a common cause of hospital-acquired infections.
- Increasing resistance to methicillin-resistant Staphylococcus aureus (MRSA) and other antibiotics complicates treatment.
- Emerging resistance necessitates updated surveillance and treatment strategies.
Purpose of the Study:
- To determine the prevalence of Staphylococcus aureus in clinical samples.
- To assess the rates of MRSA and inducible clindamycin resistance.
- To profile the full antibiotic susceptibility of Staphylococcus aureus isolates.
Main Methods:
- Standard microbiological methods were used for Staphylococcus aureus identification.
- Methicillin resistance was confirmed using cefoxitin and oxacillin disk diffusion.
- Inducible clindamycin resistance was detected using the D-zone test.
Main Results:
- 306 Staphylococcus aureus isolates were recovered from clinical samples.
- 43.1% of isolates exhibited methicillin resistance (MRSA).
- 12.4% of isolates showed inducible clindamycin resistance.
Conclusions:
- High rates of antibiotic resistance in Staphylococcus aureus clinical isolates were observed.
- The alarming rate of MRSA underscores concerns regarding antibiotic use.
- Vigilant laboratory surveillance of resistance is crucial, particularly in Nepal.
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