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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: May 1, 2026

Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants

Array Comparative Genomic Hybridization Array CGH for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

Microarray-based ultra-high resolution discovery of genomic deletion mutations.

Eric J Belfield, Carly Brown, Xiangchao Gan

¹Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK. nicholas.harberd@plants.ox.ac.uk.

|March 25, 2014

Summary

Comparative genomic hybridization (CGH) can find genome deletions, but struggles with small ones. Optimizing oligonucleotide density on microarrays improves detection of these small genomic deletions.

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Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

Oligonucleotide microarray-based comparative genomic hybridization (CGH) is a method for genome-wide deletion mutation discovery.
CGH has potential in research and diagnostics but has low power for detecting small deletions.

Purpose of the Study:

To optimize CGH for detecting genomic deletions of various sizes.
To determine the relationship between oligonucleotide density and deletion detection power.

Main Methods:

Utilized a series of Arabidopsis thaliana genomic deletion mutations (4 bp to ~5 kb).
Assessed the impact of oligonucleotide density on CGH detection of small deletions (4, 28, and 104 bp).

Main Results:

Detection of smaller deletions (4, 28, 104 bp) is dependent on oligonucleotide density.
Established necessary oligonucleotide spacings for guaranteed detection of specified deletion sizes.

Conclusions:

Findings enhance research and clinical applications of CGH.
Aids in discovering genomic deletions without prior knowledge of their location.