Related Experiment Video
Updated: May 1, 2026

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures
Published on: May 20, 2014
Diverging electrophoretic and dynamic mobility of model silica colloids at low ionic strength in ethanol
R J Kortschot1, J Lyklema2, A P Philipse1
1Van 't Hoff Laboratory for Physical and Colloid Chemistry, Debye Institute for Nanomaterials Science, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Abstract:
Electroacoustics and laser Doppler electrophoresis were employed to measure the mobility of surface-modified silica colloids in ethanol as a function of the ionic strength. Sufficiently low volume fractions were chosen to exclude effects of interparticle interactions. At high ionic strength, the electrophoretic mobility μ(e) is equal to the (electroacoustic) dynamic mobility μ(d) at 3.3 MHz. However, the ratio μ(d)/μ(e) increases significantly to ∼5 at low ionic strength. This increase may be related to the porous outer layer of the surface-modified silica spheres.
Related Concept Videos
The Colloidal State
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Electrophoresis: Overview
There...
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...

