Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

461
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
461
Bacterial Protein Maturation01:26

Bacterial Protein Maturation

630
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
630
Molecular Chaperones and Protein Folding03:00

Molecular Chaperones and Protein Folding

20.5K
The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein.
The...
20.5K
Molecular Chaperones and Protein Folding03:00

Molecular Chaperones and Protein Folding

15.2K
No description available
15.2K
Protein Transport to the Stroma01:24

Protein Transport to the Stroma

2.3K
Chloroplasts are triple membrane structures with an outer membrane, an inner membrane, and a thylakoid membrane, each containing distinct metabolite transporters, membrane translocons, and enzymes. Appropriate sorting and translocating these proteins to their correct membrane systems is essential for chloroplast function.
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
2.3K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

3.9K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Understanding residential socioeconomic and behavioral patterns for home air radon testing: a population-based intervention study in New Hampshire and Vermont (USA).

Environmental health : a global access science source·2026
Same author

Editorial Comment on "Surgical Outcome of Full-thickness Skin Graft Using Escutcheon Tissue for Management of Adult Acquired Buried Penis With Concurrent Lichen Sclerosus".

Urology·2026
Same author

Algorithmic Approach to the Management of Patients with Adult-acquired Buried Penis Syndrome.

European urology focus·2026
Same author

Floral Anatomy, Sporogenesis, and Gametogenesis in the Rubber Dandelion (<i>Taraxacum kok-saghyz</i>): Implications for Breeding and Crop Development.

Plants (Basel, Switzerland)·2026
Same author

ICG-directed scrotal flap design for adult-acquired buried penis repair.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2026
Same author

The Yeh pilus adhesin is equipped with an α-helical flap motif, which contributes to pectin adherence.

Science advances·2026

Related Experiment Video

Updated: Feb 27, 2026

Author Spotlight: Exploring Heat Shock Proteins in Malaria and Tuberculosis Infections
07:14

Author Spotlight: Exploring Heat Shock Proteins in Malaria and Tuberculosis Infections

Published on: March 8, 2024

2.0K

Chaperone function of two small heat shock proteins from maize.

Roger D Klein1, Tamutenda Chidawanyika1, Hannah S Tims1

  • 1Department of Chemistry, The College of Wooster, Wooster, OH 44691, USA.

Plant Science : an International Journal of Experimental Plant Biology
|March 25, 2014
PubMed
Summary

Two maize Class II small heat shock proteins (sHsps) were expressed and purified. Differences in their oligomerization and chaperone activity suggest distinct cellular roles, particularly during heat stress and development.

Keywords:
ChaperoneOligomerizationSmall heat shock proteinTransmission electron microscopyZea maysα-Crystallin domain

More Related Videos

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions
06:55

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

Published on: June 7, 2020

3.4K
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
08:32

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

Published on: October 23, 2016

11.1K

Related Experiment Videos

Last Updated: Feb 27, 2026

Author Spotlight: Exploring Heat Shock Proteins in Malaria and Tuberculosis Infections
07:14

Author Spotlight: Exploring Heat Shock Proteins in Malaria and Tuberculosis Infections

Published on: March 8, 2024

2.0K
Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions
06:55

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

Published on: June 7, 2020

3.4K
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
08:32

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

Published on: October 23, 2016

11.1K

Area of Science:

  • Plant molecular biology
  • Protein biochemistry
  • Cellular stress response

Background:

  • Small heat shock proteins (sHsps) are crucial molecular chaperones involved in cellular protection against various stresses.
  • While Class I sHsps are well-studied, less is known about Class II sHsps in maize.
  • Different sHsp classes are localized to distinct cellular compartments in plants.

Purpose of the Study:

  • To express and purify two Class II sHsps from Zea mays (maize).
  • To investigate the structural and functional differences between these two maize sHsps.
  • To propose distinct cellular roles for these Class II sHsps based on their properties.

Main Methods:

  • Expression and purification of two Class II sHsps from Zea mays.
  • Oligomerization analysis at different temperatures.
  • Transmission Electron Microscopy (TEM) for visualizing protein structures.
  • Chaperone activity assays using citrate synthase as a target protein.

Main Results:

  • Two Class II sHsps, ZmHsp17.0-CII and ZmHsp17.8-CII, were successfully expressed and purified.
  • Both proteins form dodecamers below heat shock temperatures; ZmHsp17.8-CII dissociates more readily at 43°C.
  • ZmHsp17.8-CII exhibited higher chaperone efficiency compared to ZmHsp17.0-CII.
  • ZmHsp17.0-CII is developmentally expressed, while ZmHsp17.8-CII is not.

Conclusions:

  • The two maize Class II sHsps, despite sequence similarity, display distinct behaviors under heat stress.
  • Differential oligomerization and chaperone activity suggest specialized functions.
  • The developmental expression pattern of ZmHsp17.0-CII further supports distinct roles within the maize cell.