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Angiotensinogen gene expression in 3T3-L1 cells
J A Saye1, L A Cassis, T W Sturgill
1Department of Pharmacology, University of Virginia, Charlottesville 22908.
The American Journal of Physiology
|February 1, 1989
Summary
Angiotensinogen messenger RNA (mRNA) is found in adipocytes and other cells. Steroid hormones appear to regulate this gene expression in fat cells.
Area of Science:
- Biochemistry
- Cell Biology
- Endocrinology
Background:
- Angiotensinogen mRNA has been detected in rat brown and white adipose tissue.
- The specific cell types responsible for angiotensinogen gene expression in adipose tissue remain unclear.
Purpose of the Study:
- To investigate angiotensinogen gene expression in adipocytes versus other cell types.
- To determine the temporal pattern of angiotensinogen mRNA expression during adipocyte differentiation.
Main Methods:
- 3T3-L1 cells were induced to differentiate into adipocytes using dexamethasone and isobutylmethylxanthine.
- RNA was extracted at various time points (0-7 days) and analyzed for angiotensinogen mRNA using a rat angiotensinogen cDNA probe.
- 3T3-C2 cells, a non-differentiating cell line, were used as a control.
Main Results:
- Angiotensinogen mRNA was readily detected in differentiated 3T3-L1 adipocytes.
- Expression was observed from days 2-7 post-drug treatment in 3T3-L1 cells.
- While angiotensinogen mRNA increased in 3T3-C2 cells upon drug treatment, the increase was greater in differentiating 3T3-L1 cells.
Conclusions:
- Angiotensinogen mRNA is present in both adipocytes and fibroblast-like cells.
- Steroid hormones appear to regulate angiotensinogen gene expression.
- Adipocytes exhibit a more pronounced induction of angiotensinogen mRNA compared to non-differentiating cells.