Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

2.2K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.2K
Experimental RNAi02:15

Experimental RNAi

6.5K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.5K
RNA Interference01:23

RNA Interference

24.3K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
24.3K
RNA Interference01:23

RNA Interference

6.4K
6.4K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

13.4K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
13.4K
Types of RNA01:20

Types of RNA

13.9K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
13.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mutations in LZTR1 drive human disease by dysregulating RAS ubiquitination.

Science (New York, N.Y.)·2018
Same author

Endoplasmic reticulum stress signaling and chemotherapy resistance in solid cancers.

Oncogenesis·2017
Same author

Compound A influences gene regulation of the Dexamethasone-activated glucocorticoid receptor by alternative cofactor recruitment.

Scientific reports·2017
Same author

Leptin receptor antagonism of iNKT cell function: a novel strategy to combat multiple myeloma.

Leukemia·2017
Same author

[How to evaluate side effects in a clinical trial?]

L'Encephale·2017
Same author

Integrated and Quantitative Proteomics of Human Tumors.

Methods in enzymology·2017

Related Experiment Video

Updated: May 1, 2026

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
09:57

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

10.4K

Getting RIDD of RNA: IRE1 in cell fate regulation.

M Maurel1, E Chevet2, J Tavernier1

  • 1Department of Medical Protein Research, VIB, Ghent, Belgium; Department of Biochemistry, Faculty of Medicine and Health Sciences, Ghent University, Ghent, Belgium.

Trends in Biochemical Sciences
|March 25, 2014
PubMed
Summary
This summary is machine-generated.

Inositol-requiring enzyme 1 (IRE1) controls cell fate through its RNA-decay (RIDD) activity. This process, distinct from XBP1 splicing, can lead to either cell survival or death during endoplasmic reticulum stress.

Keywords:
IRE1RIDDRNaseendoplasmic reticulum stressunfolded protein response

More Related Videos

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

8.5K
Generation of a RIP1 Knockout U937 Cell Line Using the CRISPR-Cas9 System
08:15

Generation of a RIP1 Knockout U937 Cell Line Using the CRISPR-Cas9 System

Published on: April 11, 2025

1.1K

Related Experiment Videos

Last Updated: May 1, 2026

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
09:57

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

10.4K
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

8.5K
Generation of a RIP1 Knockout U937 Cell Line Using the CRISPR-Cas9 System
08:15

Generation of a RIP1 Knockout U937 Cell Line Using the CRISPR-Cas9 System

Published on: April 11, 2025

1.1K

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • Inositol-requiring enzyme 1 (IRE1) is a key transducer of the unfolded protein response (UPR), crucial for maintaining proteostasis.
  • IRE1 possesses endoribonuclease activity, enabling it to generate distinct signals, including adaptive or cell death pathways.
  • These signals are mediated through unconventional splicing of XBP1 mRNA and regulated IRE1-dependent decay of mRNA (RIDD).

Purpose of the Study:

  • To review recent findings on the regulated IRE1-dependent decay (RIDD) pathway.
  • To explore the divergent activities of IRE1's RNase function in RNA processing.
  • To propose a model explaining how IRE1 RNase activity influences cell fate decisions.

Main Methods:

  • Review of existing literature on IRE1, UPR, XBP1 splicing, and RIDD.
  • Analysis of the mechanistic differences between XBP1 mRNA splicing and RIDD.
  • Development of a conceptual model for IRE1-mediated cell fate control.

Main Results:

  • RIDD cleaves RNA at XBP1-like consensus sites, but its activity differs from XBP1 splicing.
  • RIDD can contribute to either the preservation of ER homeostasis or the induction of cell death.
  • IRE1's RNase activity presents a complex regulatory mechanism for cellular responses.

Conclusions:

  • IRE1's dual function in RNA processing (splicing vs. decay) allows for nuanced control over cellular fate.
  • RIDD represents a critical, yet complex, pathway in the unfolded protein response.
  • Further research into IRE1's RNase activity is essential for understanding cell survival and death decisions under stress.